1. By demonstrating in written essay form students will show understanding of the vocabulary of definitions and structures. Biochemistry has its own language. Spend 10 to 15 minutes every day drilling on structures. At least 25% of the exam points will involve structures.
2. By demonstrating in written essay form students will show understanding of the properties of the four major classes of biochemical macromolecules. Each of these 4 categories has unique properties and characteristics. These characteristics will be emphasized to help you organize the information.
3. By demonstrating in written essay form students will show understanding of the rudiments of enzyme kinetics. Most biochemical reactions in the cell rely on catalysis, typically by protein enzymes, but also by nucleic acids. Kinetics characterizes the rates of these reactions. They allow one to understand and describe how these rates are modified by inhibitors, activators, often in cooperative interactions with the biomolecules themselves. Important coenzymes, cofactors and allosteric effectors in intermediate metabolism pathways will be described.
4. By demonstrating in written essay form students will show understanding of intermediate metabolism (glycolysis, gluconeogenesis, Krebs Cycle, oxidative phosphorylation, photosynthesis). Sequential reactions, in which the product of one reaction is passed on to act as reactant in the next, coupled reaction. The study of these interconnected pathways is metabolism. We will study the chemistry, yields and control of rates of the overall pathways by key enzymes and compounds (substrates, products, hormones, coenzymes, cofactors, etc.). Glycolysis and the Krebs Cycle are emphasized to illustrate universal principles and net energy yields.
5. By demonstrating in written essay form students will show understanding of the integratation of different aspects of biochemistry and cell biology. The frameworks of metabolism and macromolecular classification help organize the many fragments that comprise the discipline. The logic of these reactions with respect to achieving the ultimate goals of maintaining life in the cell will be emphasized to further stress the interrelationships between the subdisciplines.