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Department of Plant and Microbial Biology

http://catalog.ncsu.edu/undergraduate/collegeofals/plantbiology/

...DNA" is required for all students but BCH 454 can be substituted. Other requirements for...

BCH 454 Advanced Biochemistry Laboratory 4. Prerequisite: BCH 452, Corequisite: BCH 453.

Techniques in molecular biology and protein purification. Cloning and expression of a eukaryotic gene in bacteria followed by purification of the eukaryotic gene product. Microanalysis of DNA, RNA and protein.

PB 481 Plant Tissue Culture and Transformation 2. Prerequisite: BIT 360 or MB 409 or BCH 454 or ZO 480.

Basic techniques in plant tissue culture and transformation. Empirical approaches to techniques in plant tissue culture, designing transgenes for expression in specific plant cell organelles and tissues, use of reporter genes to optimize transformation, and troubleshooting transformation. Laboratory sessions provide hands-on experience with plant tissue culture and transformation. Use of reporter genes, fluorescence microscopy and digital imaging. Half semester course, first part.

BIT 474 Plant Genetic Engineering 2. Prerequisite: BIT 410 or BIT 510 or BCH 454 or PB 421.

This course covers fundamental hands-on techniques and strategies in plant genetic engineering. Plants are major sources of food, fiber and fuel and provide model systems for both fundamental and applied research. Students will learn techniques for stable and transient transformation of plants and plant cell cultures and selection and detection of transgene expression. Additional topics covered will include methods to generate and screen for mutants, synthetic biology and applications of plant genetic engineering. This is a half-semester course. Credit is not allowed for both BIT 474 and BIT 574.

BIT 574 Plant Genetic Engineering 2. Prerequisite: BIT 410 or BIT 510 or BCH 454 or PB 421.

This course covers fundamental hands-on techniques and strategies in plant genetic engineering. Plants are major sources of food, fiber and fuel and provide model systems for both fundamental and applied research. Students will learn techniques for stable and transient transformation of plants and plant cell cultures and selection and detection of transgene expression. Additional topics covered will include methods to generate and screen for mutants, synthetic biology and applications of plant genetic engineering. This is a half-semester course. Credit is not allowed for both BIT 474 and BIT 574.

CH 572 Proteomics 3. Prerequisite: BIT 410 or BIT 510 or BCH 454 (or approval from the instructor).

Introduction and history of the field of proteomics followed by the principles and applications of proteomics technology to understand protein expression and protein post-transitional modifications. Laboratory sessions include growing yeast with stable-isotope labeled amino acids, protein purification, Western blots, protein identification and quantification, and protein bioinformatic analysis. This is a half-semester course.

BIT 572 Proteomics 3. Prerequisite: BIT 410 or BIT 510 or BCH 454 (or approval from the instructor).

Introduction and history of the field of proteomics followed by the principles and applications of proteomics technology to understand protein expression and protein post-transitional modifications. Laboratory sessions include growing yeast with stable-isotope labeled amino acids, protein purification, Western blots, protein identification and quantification, and protein bioinformatic analysis. This is a half-semester course.

BIO 572 Proteomics 3. Prerequisite: BIT 410 or BIT 510 or BCH 454 (or approval from the instructor).

Introduction and history of the field of proteomics followed by the principles and applications of proteomics technology to understand protein expression and protein post-transitional modifications. Laboratory sessions include growing yeast with stable-isotope labeled amino acids, protein purification, Western blots, protein identification and quantification, and protein bioinformatic analysis. This is a half-semester course.

BIT 471 RNA Interference and Model Organisms 2. Prerequisite: BIT 410 or BIT 510 or BCH 454.

Introduction and history of RNA interference technology. Principles, mechanism, and applications of RNA interference in model organisms. Laboratory sessions include RNA interference-mediated silencing of genes in plants, C. elegeans, and mammalian cell culture. This is a half-semester course (8 weeks). Student may not earn credit for both BIT 471 and BIT 571.

BIT 571 RNA Interference and Model Organisms 2. Prerequisite: BIT 410 or BIT 510 or BCH 454.

Introduction and history of RNA interference technology. Principles, mechanism, and applications of RNA interference in model organisms. Laboratory sessions include RNA interference-mediated silencing of genes in plants, C. elegeans, and mammalian cell culture. This is a half-semester course (8 weeks). Student may not earn credit for both BIT 471 and BIT 571.

BIT 476 Applied Bioinformatics 2. Prerequisite: BIT 410 or BCH 454 or GN 311.

The haploid human genome occupies a total of just over 3 billion DNA base pairs. This information is not contained in books, but stored in electronic databases. Computational biology utilizes infer function by comparative analysis. This course is designed for life scientists from all fields to introduce them to the power of bioinformatics and enable them to access and utilize biological information in databases for their own research.

BIT 468 Genome Mapping 2. Prerequisite: BIT 410 or BIT 510 or BCH 454.

Students will be introduced to basic techniques in genetic and physical mapping. The principles of DNA marker development, marker detection, genetic and physical mapping and DNA sequencing will be addressed from a practical view with an emphasis onagricultural applications. This is a half semester course. Student must register for both lecture and lab sections.

BIT 568 Genome Mapping 2. Prerequisite: BIT 410 or BIT 510 or BCH 454.

Students will be introduced to basic techniques in genetic and physical mapping. The principles of DNA marker development, marker detection, genetic and physical mapping and DNA sequencing will be addressed from a practical view with an emphasis onagricultural applications. This is a half semester course. Student must register for both lecture and lab sections.

ANS 454 Lactation, Milk and Nutrition 3. Prerequisite: ANS 230 or FS/NTR 400; BCH 451 or ZO 421.

Nutritional properties of milk as a high-quality food with nutritional diversity. Principles of physiology, biochemistry and cell biology in the mammary gland. Procedures of milk production and milk collection for milk quality and nutrition. Human lactation vs. that of domestic animals. Impacts of biotechnology and food safety on dairy production. Credit will not be given for both ANS 454 and 554.

NTR 454 Lactation, Milk and Nutrition 3. Prerequisite: ANS 230 or FS/NTR 400; BCH 451 or ZO 421.

Nutritional properties of milk as a high-quality food with nutritional diversity. Principles of physiology, biochemistry and cell biology in the mammary gland. Procedures of milk production and milk collection for milk quality and nutrition. Human lactation vs. that of domestic animals. Impacts of biotechnology and food safety on dairy production. Credit will not be given for both ANS 454 and 554.

BIT 464 Protein Purification 2. Prerequisite: BIT 410 or BIT 510 or BCH 454.

Comparison of several different chromatography techniques for protein purification. Construction of purification tables and SDS-and native-PAGE analysis. Cost-benefit analysis of industrial-scale procedures. Half semester course, second part.

BIT 466 Animal Cell Culture Techniques 2. Prerequisite: BIT 410 or BIT 510 or BCH 454.

Introduction to animal cell culture techniques. Aseptic technique for vertebrate cell culture, media formulation, primary cell culture, long-term maintenance of cell lines, application of molecular techniques to in vitro situations. Half semester course, first part.

PB 480 Introduction to Plant Biotechnology 3. Prerequisite: BCH 454 or BIT 410 or CS 211 or GN 311 or PB/BIO 414 or PB 421..

Introduction to gene cloning, plant tissue culture and transformation, and the development of agriculturally important transgenic traits. Critical thinking, case studies, and discussions are used to examine global approaches to the regulation and risks of genetically-modified organisms, plant and gene patents, and the consequences of these factors on food soverienty and trade. Students cannot receive credit for both PB 480 and PB 580.

PB 580 Introduction to Plant Biotechnology 3. Prerequisite: BCH 454 or BIT 410 or CS 211 or GN 311 or PB/BIO 414 or PB 421.

Introduction to gene cloning, plant tissue culture and transformation, and the development of agriculturally important transgenic traits. Critical thinking, case studies, and discussions are used to examine global approaches to the regulation and risks of genetically-modified organisms, plant and gene patents, and the consequences of these factors on food soverienty and trade. Students cannot receive credit for both PB 480 and PB 580.

BIT 573 Protein-Protein Interactions 2. Prerequisite: BIT 410 or BIT 510 or BCH 454 (or approval from the instructor).

The interactions of proteins mediate numerous biological processes of cells. This course focuses on ways to identify and study protein-protein interactions. Students will apply a variety of methods for studying protein-protein interactions, focusing on the advantages and limitations of each technique and how to apply the methods in a laboratory setting. In lab, students will perform a yeast two-hybrid experiment and a co-immunoprecipitation from proteins expressed in mammalian cell culture to confirm detected interactions. This is a half-semester course.

BIT 473 Protein Interactions 2. Prerequisite: BIT 410 or BCH 454.

The interactions of proteins mediate numerous biological processes of cells. This course focuses on ways to identify and study protein-protein interactions, focusing on the advantages and limitations of each technique and how to apply the methods in a laboratory setting. In lab, students will perform a yeast two-hybrid experiment and a co-immunoprecipitation from proteins expressed in mammalian cell culture to confirm detected interactions. This a half-semester course.