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Department of Plant and Microbial Biology

http://catalog.ncsu.edu/undergraduate/collegeofals/plantbiology/

...with a grade of C- or better. BIT 410 , "Manipulation of Recombinant DNA" is required...

BIT 569 RNA Purification and Analysis 2. Prerequisite: BIT 410 or 510.

Laboratory-intensive course covering techniques in RNA purification and analysis including: isolation of quality RNA; quantification by gel and spectrophotometer; separation by gel electrophoresis; reverse transcription PCR; and Northern blotting using non-radioactive labeling and detection by chemi-luminescense. Half semester course.

BIT 474 Plant Genetic Engineering 2. Prerequisite: BIT 410 or BIT 510 or BCH 454 or PB 421.

This course covers fundamental hands-on techniques and strategies in plant genetic engineering. Plants are major sources of food, fiber and fuel and provide model systems for both fundamental and applied research. Students will learn techniques for stable and transient transformation of plants and plant cell cultures and selection and detection of transgene expression. Additional topics covered will include methods to generate and screen for mutants, synthetic biology and applications of plant genetic engineering. This is a half-semester course. Credit is not allowed for both BIT 474 and BIT 574.

BIT 574 Plant Genetic Engineering 2. Prerequisite: BIT 410 or BIT 510 or BCH 454 or PB 421.

This course covers fundamental hands-on techniques and strategies in plant genetic engineering. Plants are major sources of food, fiber and fuel and provide model systems for both fundamental and applied research. Students will learn techniques for stable and transient transformation of plants and plant cell cultures and selection and detection of transgene expression. Additional topics covered will include methods to generate and screen for mutants, synthetic biology and applications of plant genetic engineering. This is a half-semester course. Credit is not allowed for both BIT 474 and BIT 574.

BIT 410 Manipulation of Recombinant DNA 4. Prerequisite: BIO 183 or ZO/BIO 160 and CH 223 or CH 227 with a C- or better.

Introduction to molecular biology and protein chemistry. Theory behind laboratory techniques and overview of cloning strategies starting from nucleic acid or protein sequence data. Laboratory sessions involve subcloning, preparation of competent cells, transformation, screening recombinant DNA by colony hybridization and PCR, SDS-PAGE of recombinant protein, affinity purification, and western blots.

BIT 477 Metagenomics 2. Prerequisite: BIT 410 or 510.

Participants will be introduced to a variety of methods for studying the complex microbial populations that surround us, including theory, applications, limitations, and health and legal implications. Students will apply deep sequencing techniques to mine the genetic diversity of complex microbial populations such as the rhizosphere, a swine lagoon sample, or even the communities of microbes growing happily inside your kitchen sink drain. This course will provide hands-on experience with molecular and computational tools that can be used to study the relationships between microbial communities and ecosystems or hosts.

BIT 478 Mapping the Brain 2. Prerequisite: BIT 410.

Mapping the Brain is designed to provide students with a neuroscience research experience. In lecture, students will gain an appreciation for the fundamental challenges inherent in studying the brain and explore the theory, applications, and limitations of new and traditional technologies employed in modern neuroscience. In the lab, students will use a novel transgenic mouse model to analyze the connections of a single population of neurons and the effects of stimulating their activity in vivo. This hands-on laboratory research experience will expose students to common laboratory approaches (histology, microscopy, etc.) as well as to cutting edge genetic approaches.

CH 572 Proteomics 3. Prerequisite: BIT 410 or BIT 510 or BCH 454 (or approval from the instructor).

Introduction and history of the field of proteomics followed by the principles and applications of proteomics technology to understand protein expression and protein post-transitional modifications. Laboratory sessions include growing yeast with stable-isotope labeled amino acids, protein purification, Western blots, protein identification and quantification, and protein bioinformatic analysis. This is a half-semester course.

BIT 572 Proteomics 3. Prerequisite: BIT 410 or BIT 510 or BCH 454 (or approval from the instructor).

Introduction and history of the field of proteomics followed by the principles and applications of proteomics technology to understand protein expression and protein post-transitional modifications. Laboratory sessions include growing yeast with stable-isotope labeled amino acids, protein purification, Western blots, protein identification and quantification, and protein bioinformatic analysis. This is a half-semester course.

BIO 572 Proteomics 3. Prerequisite: BIT 410 or BIT 510 or BCH 454 (or approval from the instructor).

Introduction and history of the field of proteomics followed by the principles and applications of proteomics technology to understand protein expression and protein post-transitional modifications. Laboratory sessions include growing yeast with stable-isotope labeled amino acids, protein purification, Western blots, protein identification and quantification, and protein bioinformatic analysis. This is a half-semester course.

BIT 471 RNA Interference and Model Organisms 2. Prerequisite: BIT 410 or BIT 510 or BCH 454.

Introduction and history of RNA interference technology. Principles, mechanism, and applications of RNA interference in model organisms. Laboratory sessions include RNA interference-mediated silencing of genes in plants, C. elegeans, and mammalian cell culture. This is a half-semester course (8 weeks). Student may not earn credit for both BIT 471 and BIT 571.

BIT 571 RNA Interference and Model Organisms 2. Prerequisite: BIT 410 or BIT 510 or BCH 454.

Introduction and history of RNA interference technology. Principles, mechanism, and applications of RNA interference in model organisms. Laboratory sessions include RNA interference-mediated silencing of genes in plants, C. elegeans, and mammalian cell culture. This is a half-semester course (8 weeks). Student may not earn credit for both BIT 471 and BIT 571.

BIT 465 Real-time PCR Techniques 2. Prerequisite: BIT 410 or 510.

Real time PCR is an evolving technique with its basis in the dynamic properties of the polymerase chain reaction and fluorescent detection. We will review current real-time theory, techniques, machinery, troubleshooting, tools, and advanced protocols for sequence detection including SYBR green, TaqMan, Beacons, multiplexing, and single nucleotide polymorphism analysis. Students will have the opportunity to utilize skills learned during lecture in a laboratory environment. At the conclusion of this course, students should feel comfortable with real-time experimental design, its tools, and analysis of generated data. This is a half-semester course. Student must register for both lecture and lab sections.

BIT 565 Real-time PCR Techniques 2. Prerequisite: BIT 410 or 510.

Real time PCR is an evolving technique with its basis in the dynamic properties of the polymerase chain reaction and fluorescent detection. We will review current real-time theory, techniques, machinery, troubleshooting, tools, and advanced protocols for sequence detection including SYBR green, TaqMan, Beacons, multiplexing, and single nucleotide polymorphism analysis. Students will have the opportunity to utilize skills learned during lecture in a laboratory environment. At the conclusion of this course, students should feel comfortable with real-time experimental design, its tools, and analysis of generated data. This is a half-semester course. Student must register for both lecture and lab sections.

BEC 541 Expression Systems in Biomanufacturing II 3. Prerequisite: BEC 440/540 or BIT 410/510.

Introduction to insect and mammalian cell expression systems, their advantages and disadvantages. Advanced techniques in DNA cloning, cell transformation, and optimization of protein expression. Selection, archiving and characterization of production line. The lab portion of the course provides students with practical experience in DNA cloning and protein expression techniques in insect and mammalian cell expression systems. Credit not given for both.

BEC 441 Expression Systems in Biomanufacturing II 3. Prerequisite: BEC 440/540 or BIT 410/510.

Introduction to insect and mammalian cell expression systems, their advantages and disadvantages. Advanced techniques in DNA cloning, cell transformation, and optimization of protein expression. Selection, archiving and characterization of production line. The lab portion of the course provides students with practical experience in DNA cloning and protein expression techniques in insect and mammalian cell expression systems. Credit not given for both.

BIO 482 Capstone Course in Molecular, Cellular, and Developmental Biology 3. Prerequisite: C- or better in BIO 361, BIO/PB 414, and one of the following: BCH 351 or BCH 451 or BIT 410 or GN 311..

Topical problems in molecular, cellular, and developmental biology. BIO 482 provides a challenging opportunity for students to integrate and apply knowledge and skills gained from their major studies. Emphasis will be placed on collaborative learning and on effective, professional communication. Topics and instructors will vary from semester to semester. Priority will initially be given to seniors in the MCD curriculum; other students with the necessary prerequisites will be admitted on a space available basis.

BIT 467 PCR and DNA Fingerprinting 2. Prerequisite: BIT 410/510.

Introduction to polymerase chain reaction. Optimization of PCR reactions and primer design for DNA sequences using DNA databases available on the web. Laboratory sections include using rapid techniques for isolating and sequencing DNA from small amounts of sample and forensic identification of individuals using isolated human hairs. Credit is not allowed for both BIT 467 and BIT 567.

BIT 462 Gene Expression Analysis: Microarrays 2. Prerequisite: BIT 410 or 510.

Microarray analysis is an evolving technique with its basis in the dynamic properties of the nucleic acid hybridization. We will review current theory, techniques, instrumentation, troubleshooting, analysis tools, and advanced protocols for microarray analysis. Students will have the opportunity to utilize skills learned during lecture in a laboratory environment and have access to exceptional instrumentation. At the conclusion of this course, students should feel comfortable with microarray experimental design, its tools, an analysis of generated data. This is a half-semester course. Student must register for both lecture and lab sections.

BIT 562 Gene Expression Analysis: Microarrays 2. Prerequisite: BIT 410 or 510.

Microarray analysis is an evolving technique with its basis in the dynamic properties of the nucleic acid hybridization. We will review current theory, techniques, instrumentation, troubleshooting, analysis tools, and advanced protocols for microarray analysis. Students will have the opportunity to utilize skills learned during lecture in a laboratory environment and have access to exceptional instrumentation. At the conclusion of this course, students should feel comfortable with microarray experimental design, its tools, an analysis of generated data. This is a half-semester course. Student must register for both lecture and lab sections.

BIT 476 Applied Bioinformatics 2. Prerequisite: BIT 410 or BCH 454 or GN 311.

The haploid human genome occupies a total of just over 3 billion DNA base pairs. This information is not contained in books, but stored in electronic databases. Computational biology utilizes infer function by comparative analysis. This course is designed for life scientists from all fields to introduce them to the power of bioinformatics and enable them to access and utilize biological information in databases for their own research.

BIT 577 Metagenomics 2. Prerequisite: BIT 410 or 510.

Participants will be introduced to a variety of methods for studying the complex microbial populations that surround us, including theory, applications, limitations, and health and legal implications. Students will apply deep sequencing techniques to mine the genetic diversity of complex microbial populations such as the rhizosphere, a swine lagoon sample, or even the communities of microbes growing happily inside your kitchen sink drain. This course will provide hands-on experience with molecular and computational tools that can be used to study the relationships between microbial communities and ecosystems or hosts.

CHE 463 Fermentation of Recombinant Microorganisms 2. Prerequisite: CH 223 or CH 227; Corequisite: (BIT 410 or BCH 452 or MB 352 or BEC 363).

Introduction to fermentation and protein chemistry. Theory behind laboratory techniques and overview of industrial scale expression systems. Laboratory sessions involve use of microbial expression vectors, fermentation systems, and large-scale purification of recombinant protein. Half semester course, first part.

BEC 463 Fermentation of Recombinant Microorganisms 2. Prerequisite: CH 223 or CH 227; Corequisite: (BIT 410 or BCH 452 or MB 352 or BEC 363).

Introduction to fermentation and protein chemistry. Theory behind laboratory techniques and overview of industrial scale expression systems. Laboratory sessions involve use of microbial expression vectors, fermentation systems, and large-scale purification of recombinant protein. Half semester course, first part.

BIT 468 Genome Mapping 2. Prerequisite: BIT 410 or BIT 510 or BCH 454.

Students will be introduced to basic techniques in genetic and physical mapping. The principles of DNA marker development, marker detection, genetic and physical mapping and DNA sequencing will be addressed from a practical view with an emphasis onagricultural applications. This is a half semester course. Student must register for both lecture and lab sections.

BIT 568 Genome Mapping 2. Prerequisite: BIT 410 or BIT 510 or BCH 454.

Students will be introduced to basic techniques in genetic and physical mapping. The principles of DNA marker development, marker detection, genetic and physical mapping and DNA sequencing will be addressed from a practical view with an emphasis onagricultural applications. This is a half semester course. Student must register for both lecture and lab sections.

BIT 464 Protein Purification 2. Prerequisite: BIT 410 or BIT 510 or BCH 454.

Comparison of several different chromatography techniques for protein purification. Construction of purification tables and SDS-and native-PAGE analysis. Cost-benefit analysis of industrial-scale procedures. Half semester course, second part.

BIT 466 Animal Cell Culture Techniques 2. Prerequisite: BIT 410 or BIT 510 or BCH 454.

Introduction to animal cell culture techniques. Aseptic technique for vertebrate cell culture, media formulation, primary cell culture, long-term maintenance of cell lines, application of molecular techniques to in vitro situations. Half semester course, first part.

PB 480 Introduction to Plant Biotechnology 3. Prerequisite: BCH 454 or BIT 410 or CS 211 or GN 311 or PB/BIO 414 or PB 421..

Introduction to gene cloning, plant tissue culture and transformation, and the development of agriculturally important transgenic traits. Critical thinking, case studies, and discussions are used to examine global approaches to the regulation and risks of genetically-modified organisms, plant and gene patents, and the consequences of these factors on food soverienty and trade. Students cannot receive credit for both PB 480 and PB 580.

PB 580 Introduction to Plant Biotechnology 3. Prerequisite: BCH 454 or BIT 410 or CS 211 or GN 311 or PB/BIO 414 or PB 421.

Introduction to gene cloning, plant tissue culture and transformation, and the development of agriculturally important transgenic traits. Critical thinking, case studies, and discussions are used to examine global approaches to the regulation and risks of genetically-modified organisms, plant and gene patents, and the consequences of these factors on food soverienty and trade. Students cannot receive credit for both PB 480 and PB 580.

BIT 573 Protein-Protein Interactions 2. Prerequisite: BIT 410 or BIT 510 or BCH 454 (or approval from the instructor).

The interactions of proteins mediate numerous biological processes of cells. This course focuses on ways to identify and study protein-protein interactions. Students will apply a variety of methods for studying protein-protein interactions, focusing on the advantages and limitations of each technique and how to apply the methods in a laboratory setting. In lab, students will perform a yeast two-hybrid experiment and a co-immunoprecipitation from proteins expressed in mammalian cell culture to confirm detected interactions. This is a half-semester course.

BIT 473 Protein Interactions 2. Prerequisite: BIT 410 or BCH 454.

The interactions of proteins mediate numerous biological processes of cells. This course focuses on ways to identify and study protein-protein interactions, focusing on the advantages and limitations of each technique and how to apply the methods in a laboratory setting. In lab, students will perform a yeast two-hybrid experiment and a co-immunoprecipitation from proteins expressed in mammalian cell culture to confirm detected interactions. This a half-semester course.