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BIT 569 RNA Purification and Analysis 2. Prerequisite: BIT 410 or 510.

Laboratory-intensive course covering techniques in RNA purification and analysis including: isolation of quality RNA; quantification by gel and spectrophotometer; separation by gel electrophoresis; reverse transcription PCR; and Northern blotting using non-radioactive labeling and detection by chemi-luminescense. Half semester course.

BIT 474 Plant Genetic Engineering 2. Prerequisite: BIT 410 or BIT 510 or BCH 454 or PB 421.

This course covers fundamental hands-on techniques and strategies in plant genetic engineering. Plants are major sources of food, fiber and fuel and provide model systems for both fundamental and applied research. Students will learn techniques for stable and transient transformation of plants and plant cell cultures and selection and detection of transgene expression. Additional topics covered will include methods to generate and screen for mutants, synthetic biology and applications of plant genetic engineering. This is a half-semester course. Credit is not allowed for both BIT 474 and BIT 574.

BIT 574 Plant Genetic Engineering 2. Prerequisite: BIT 410 or BIT 510 or BCH 454 or PB 421.

This course covers fundamental hands-on techniques and strategies in plant genetic engineering. Plants are major sources of food, fiber and fuel and provide model systems for both fundamental and applied research. Students will learn techniques for stable and transient transformation of plants and plant cell cultures and selection and detection of transgene expression. Additional topics covered will include methods to generate and screen for mutants, synthetic biology and applications of plant genetic engineering. This is a half-semester course. Credit is not allowed for both BIT 474 and BIT 574.

BIT 815 Advanced Special Topics 1-6. Prerequisite: BIT 510.

Intensive three-week or six-week courses in advanced technologies such as DNA sequencing, downstream processing, immunological techniques, construction of c-DNA libraries, mammalian embryo manipulation, plant transformation, bioreactor design, cloning in gram positive bacteria, electron microscopy or techniques in yeast molecular biology.

BIT 477 Metagenomics 2. Prerequisite: BIT 410 or 510.

Participants will be introduced to a variety of methods for studying the complex microbial populations that surround us, including theory, applications, limitations, and health and legal implications. Students will apply deep sequencing techniques to mine the genetic diversity of complex microbial populations such as the rhizosphere, a swine lagoon sample, or even the communities of microbes growing happily inside your kitchen sink drain. This course will provide hands-on experience with molecular and computational tools that can be used to study the relationships between microbial communities and ecosystems or hosts.

BIT 578 Mapping the Brain 2. Prerequisite: BIT 510.

Mapping the Brain is designed to provide students with a neuroscience research experience. In lecture, students will gain an appreciation for the fundamental challenges inherent in studying the brain and explore the theory, applications, and limitations of new and traditional technologies employed in modern neuroscience. In the lab, students will use a novel transgenic mouse model to analyze the connections of a single population of neurons and the effects of stimulating their activity in vivo. This hands-on laboratory research experience will expose students to common laboratory approaches (histology, microscopy, etc.) as well as to cutting edge genetic approaches.

CH 572 Proteomics 3. Prerequisite: BIT 410 or BIT 510 or BCH 454 (or approval from the instructor).

Introduction and history of the field of proteomics followed by the principles and applications of proteomics technology to understand protein expression and protein post-transitional modifications. Laboratory sessions include growing yeast with stable-isotope labeled amino acids, protein purification, Western blots, protein identification and quantification, and protein bioinformatic analysis. This is a half-semester course.

BIT 572 Proteomics 3. Prerequisite: BIT 410 or BIT 510 or BCH 454 (or approval from the instructor).

Introduction and history of the field of proteomics followed by the principles and applications of proteomics technology to understand protein expression and protein post-transitional modifications. Laboratory sessions include growing yeast with stable-isotope labeled amino acids, protein purification, Western blots, protein identification and quantification, and protein bioinformatic analysis. This is a half-semester course.

BIO 572 Proteomics 3. Prerequisite: BIT 410 or BIT 510 or BCH 454 (or approval from the instructor).

Introduction and history of the field of proteomics followed by the principles and applications of proteomics technology to understand protein expression and protein post-transitional modifications. Laboratory sessions include growing yeast with stable-isotope labeled amino acids, protein purification, Western blots, protein identification and quantification, and protein bioinformatic analysis. This is a half-semester course.

BIT 471 RNA Interference and Model Organisms 2. Prerequisite: BIT 410 or BIT 510 or BCH 454.

Introduction and history of RNA interference technology. Principles, mechanism, and applications of RNA interference in model organisms. Laboratory sessions include RNA interference-mediated silencing of genes in plants, C. elegeans, and mammalian cell culture. This is a half-semester course (8 weeks). Student may not earn credit for both BIT 471 and BIT 571.

BIT 571 RNA Interference and Model Organisms 2. Prerequisite: BIT 410 or BIT 510 or BCH 454.

Introduction and history of RNA interference technology. Principles, mechanism, and applications of RNA interference in model organisms. Laboratory sessions include RNA interference-mediated silencing of genes in plants, C. elegeans, and mammalian cell culture. This is a half-semester course (8 weeks). Student may not earn credit for both BIT 471 and BIT 571.

BIT 465 Real-time PCR Techniques 2. Prerequisite: BIT 410 or 510.

Real time PCR is an evolving technique with its basis in the dynamic properties of the polymerase chain reaction and fluorescent detection. We will review current real-time theory, techniques, machinery, troubleshooting, tools, and advanced protocols for sequence detection including SYBR green, TaqMan, Beacons, multiplexing, and single nucleotide polymorphism analysis. Students will have the opportunity to utilize skills learned during lecture in a laboratory environment. At the conclusion of this course, students should feel comfortable with real-time experimental design, its tools, and analysis of generated data. This is a half-semester course. Student must register for both lecture and lab sections.

BIT 565 Real-time PCR Techniques 2. Prerequisite: BIT 410 or 510.

Real time PCR is an evolving technique with its basis in the dynamic properties of the polymerase chain reaction and fluorescent detection. We will review current real-time theory, techniques, machinery, troubleshooting, tools, and advanced protocols for sequence detection including SYBR green, TaqMan, Beacons, multiplexing, and single nucleotide polymorphism analysis. Students will have the opportunity to utilize skills learned during lecture in a laboratory environment. At the conclusion of this course, students should feel comfortable with real-time experimental design, its tools, and analysis of generated data. This is a half-semester course. Student must register for both lecture and lab sections.

BEC 541 Expression Systems in Biomanufacturing II 3. Prerequisite: BEC 440/540 or BIT 410/510.

Introduction to insect and mammalian cell expression systems, their advantages and disadvantages. Advanced techniques in DNA cloning, cell transformation, and optimization of protein expression. Selection, archiving and characterization of production line. The lab portion of the course provides students with practical experience in DNA cloning and protein expression techniques in insect and mammalian cell expression systems. Credit not given for both.

BEC 441 Expression Systems in Biomanufacturing II 3. Prerequisite: BEC 440/540 or BIT 410/510.

Introduction to insect and mammalian cell expression systems, their advantages and disadvantages. Advanced techniques in DNA cloning, cell transformation, and optimization of protein expression. Selection, archiving and characterization of production line. The lab portion of the course provides students with practical experience in DNA cloning and protein expression techniques in insect and mammalian cell expression systems. Credit not given for both.

BIT 467 PCR and DNA Fingerprinting 2. Prerequisite: BIT 410/510.

Introduction to polymerase chain reaction. Optimization of PCR reactions and primer design for DNA sequences using DNA databases available on the web. Laboratory sections include using rapid techniques for isolating and sequencing DNA from small amounts of sample and forensic identification of individuals using isolated human hairs. Credit is not allowed for both BIT 467 and BIT 567.

BIT 462 Gene Expression Analysis: Microarrays 2. Prerequisite: BIT 410 or 510.

Microarray analysis is an evolving technique with its basis in the dynamic properties of the nucleic acid hybridization. We will review current theory, techniques, instrumentation, troubleshooting, analysis tools, and advanced protocols for microarray analysis. Students will have the opportunity to utilize skills learned during lecture in a laboratory environment and have access to exceptional instrumentation. At the conclusion of this course, students should feel comfortable with microarray experimental design, its tools, an analysis of generated data. This is a half-semester course. Student must register for both lecture and lab sections.

BIT 562 Gene Expression Analysis: Microarrays 2. Prerequisite: BIT 410 or 510.

Microarray analysis is an evolving technique with its basis in the dynamic properties of the nucleic acid hybridization. We will review current theory, techniques, instrumentation, troubleshooting, analysis tools, and advanced protocols for microarray analysis. Students will have the opportunity to utilize skills learned during lecture in a laboratory environment and have access to exceptional instrumentation. At the conclusion of this course, students should feel comfortable with microarray experimental design, its tools, an analysis of generated data. This is a half-semester course. Student must register for both lecture and lab sections.

BIT 577 Metagenomics 2. Prerequisite: BIT 410 or 510.

Participants will be introduced to a variety of methods for studying the complex microbial populations that surround us, including theory, applications, limitations, and health and legal implications. Students will apply deep sequencing techniques to mine the genetic diversity of complex microbial populations such as the rhizosphere, a swine lagoon sample, or even the communities of microbes growing happily inside your kitchen sink drain. This course will provide hands-on experience with molecular and computational tools that can be used to study the relationships between microbial communities and ecosystems or hosts.

BIT 510 Core Technologies in Molecular and Cellular Biology 4. Prerequisite: Equivalent of CH 223 and (MB 351 or GN 311).

Basic technologies of recombinant DNA procedures, gene expression, isolation and identification of nucleic acids and proteins.

BIT 468 Genome Mapping 2. Prerequisite: BIT 410 or BIT 510 or BCH 454.

Students will be introduced to basic techniques in genetic and physical mapping. The principles of DNA marker development, marker detection, genetic and physical mapping and DNA sequencing will be addressed from a practical view with an emphasis onagricultural applications. This is a half semester course. Student must register for both lecture and lab sections.

BIT 568 Genome Mapping 2. Prerequisite: BIT 410 or BIT 510 or BCH 454.

Students will be introduced to basic techniques in genetic and physical mapping. The principles of DNA marker development, marker detection, genetic and physical mapping and DNA sequencing will be addressed from a practical view with an emphasis onagricultural applications. This is a half semester course. Student must register for both lecture and lab sections.

BIT 464 Protein Purification 2. Prerequisite: BIT 410 or BIT 510 or BCH 454.

Comparison of several different chromatography techniques for protein purification. Construction of purification tables and SDS-and native-PAGE analysis. Cost-benefit analysis of industrial-scale procedures. Half semester course, second part.

BIT 466 Animal Cell Culture Techniques 2. Prerequisite: BIT 410 or BIT 510 or BCH 454.

Introduction to animal cell culture techniques. Aseptic technique for vertebrate cell culture, media formulation, primary cell culture, long-term maintenance of cell lines, application of molecular techniques to in vitro situations. Half semester course, first part.

BIT 573 Protein-Protein Interactions 2. Prerequisite: BIT 410 or BIT 510 or BCH 454 (or approval from the instructor).

The interactions of proteins mediate numerous biological processes of cells. This course focuses on ways to identify and study protein-protein interactions. Students will apply a variety of methods for studying protein-protein interactions, focusing on the advantages and limitations of each technique and how to apply the methods in a laboratory setting. In lab, students will perform a yeast two-hybrid experiment and a co-immunoprecipitation from proteins expressed in mammalian cell culture to confirm detected interactions. This is a half-semester course.